AEBSF hydrochloride (Pefabloc SC) is an irreversible inhibitor of serine proteases, such as chymotrypsin, kallikrein, thrombin, plasmin, and trypsin.

在蟑螂诱发的小鼠过敏模型中,AEBSF能够减少其呼吸道反应和潜在的炎症.在小鼠中注射致死量的弓形虫,每天腹腔注射76.8 mg/kg AEBSF,能够延长其生存期.

AEBSF是一种丝氨酸蛋白酶抑制剂,它能够抑制巨噬细胞裂解白血病细胞,并且不会抑制巨噬细胞分泌TNF-α和IL-1β。在5种不同人细胞系中,AEBSF通过抑制神经和非神经中的β分泌,从而抑制组成Aβ的产物。AEBSF能够扰乱子宫内膜中囊胚的生长,还能够改变蛋白分泌机制从而抑制在人脐静脉内皮细胞中HeLa细胞的粘附。

The HeLa cells suspended in RPMI-1640 media containing 10% FCS are plated into each well of a 96-well microplate (5×103 cells/200 μL/well). After incubation for 24 h at 37°C, cells are treated with different doses of AEBSF (0, 25, 50, 100 μg/mL) for 48 h. Then, 20 μL fresh 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-diphenytetrazoliumromide (MTT) reagent (5 μg/μL) is added into each well, and cells are cultured at 37°C in 5% CO2 for another 4 h. The media are discarded carefully, and 150 μL DMSO is added. Absorbance is read on a microplate reader at dual wavelengths of 540 and 620 nm.